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EQUIPMENT
UV-Vis-NIR Spectrophotometer, UV-3600 type, Shimadzu, with integrating sphere attachment
UV-Vis Spectrophotometer, Lambda 35 type, Perkin Elmer, with the constant-temperature cell holder and Peltier thermostat, and with integrating sphere attachment
UV-Vis Spectrophotometer, UV-2100 type, Shimadzu, with the constant-temperature cell holder and Peltier thermostat
UV-Vis Spectrophotometer, Lambda 12 type, Perkin Elmer
UV-Vis Spectrophotometer, 8453 Diode Array type, Hewlett Packard
Fluorescence Spectrometer LS 55, Perkin Elmer
- measurements modes:
- fluorescence
- phosphorescence
- chemiluminescence
- bioluminescence
- excitation range: 200-800 nm
- emission detection range: 200-900 nm
- the constant-temperature cell holder
Stopped-flow Spectrometer SX-17MV, Applied Photophysics
- absorbance and fluorescence detection by Diode Array system
- temperature control by Refrigerated Circulators Grant LTD6G series, Grant Instruments (temp. range from -30°C to +100°C)
- features:
- dead-time cell ca. 1 ms
- optical path lengths of 10 mm and 2 mm
- flow circuit materials suitable for anaerobic experiments
- adjustable sample volume
Light sources:
- High pressure mercury lamps HBO 500, HBO 200 and high pressure xenone lamps XBO150, XBO75 with power suppliers
- diode laser delivered 100 mW at 748 nm, Hamamatsu, type LA0873, S/N M070301 controlled by a ThorLabs 500 mA ACC/APC Laser Diode Controller
Laser Flash Photolysis Spectrometer, type LKS.60, Applied Photophysics1. Laser excitation flash:
- Wavelength: 532 nm, 355 nm or 266 nm;
- Single pulse maximal energy, respectively: 245 mJ, 100 mJ or 75 mJ;
- Momentary pulse power, respectively : 50 MW, 20 MW or 15 MW;
- Pulsewidth (full width half maximum): 4 - 6 ns;
- Laser beam diameter: 6 mm.
2. Detection:
- Detection light source: high-pressure xenon arc lamp 150 W/CR;
- Arc lamp pulser: 400 μs plateau region, increase of light energy from 50 to 150 times.
- Scanning wavelength range: 200 nm – 1000 nm or 900 nm – 1400 nm;
- Number of measurement points (after single excitation flash): 512;
- Minimal time period between data points: 0,5 ns;
- Maximal time period between data points: 2 ms;
- Accuracy of measurement: 0,002 absorbance unit;
Microsecond classical flash photolysis system
1. Excitation flash:
- Two 1000 J xenon flash lamps. Polychromatic pulse irradiation with cut-off filter modifier;
- Momentary pulse power: 20 MW. Pulsewidth (full width half maximum): 25 μs;
2. Detection with photomultiplier and A/D converter (PCL 818 HD, Advantech):
- Detection light source: deuterium D2E or tungsten lamp;
- Wavelength: 220 nm – 850 nm;
- Number of measurement points in one packet: 100;
- Time period between data points in packet: 10 μs
- Number of measurement packets: 1 - 4000;
- Time period between packets: 0 – 150 ms ;
- Maximal measurement time: 10 min;
- Lamps: XBO150 and XBO75
- Electrochemical analyzers: BAS CV50W, mtm-anko M161
- Optel, Automatic monochromator
- Pico Technology, PicoScop 2203 Oscilloscope
- TTi, Function Generator TG550
- TTi, Wideband Amplifier WA301
- Hewlett Packard, UV-Vis 8453 Diode Array Spectrometer,
The equipment enabling- working under inert gas conditions
- measurements using various techniques (CV, LSV, DPV, OSWV)
- configuration of instruments for different type of measurements
- measurement of flat band potential in photostatic conditions
- possibility of light modulation
- modulation spectroscopy
Light Engine UNO 50:
- 375 nm (to 40 mW)
- 405 nm (to 155 mW)
- 465 nm (to 165 mW)
- 520 nm (to 85 mW)
- 595 nm (to 47 mW)
- 630 nm (to 165 mW)
- 870 nm (to 108 mW)
Equipment is applied for electrophoretic separation of proteins and nucleic acids in polyacrylamide or agarose gels in vertical or horizontal systems.
- Electrophoretic separation can be carried out in:
- denaturing conditions (SDS-PAGE)
- native conditions (PAGE)
HPLC instrument, Perkin Elmer Series 200 LC
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High pressure liquid chromatograph (HPLC) instrument equipped with:
- pump quaternary LC solvent delivery
- 4-channel vacuum degassing package
- sample loop for 10-port valve, volume 20 and 100 ml
- UV/Vis detector
- enabling measurements in pressure range from 0,1 to 250 MPa
- can be combined with:
- UV-Vis-NIR spectrophotometer
- laser flash photolysis spectrometer
- fluorescence spectrometer
- Synthesis in various gas environments (oxygen, argon, nitrogen, CO2, NO)
- Manipulation under vacuum
- Microwave synthesis
